Anticorps Recombinant de lapin anti-Siglec-9

Siglec-9 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241799C10

N° de cat : CL488-98264-6

Synonymes

SIGLEC9, 241799C10, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using CL488-98264-6

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Siglec-9 Rabbit RecAb (CL488-98264-6, Clone: 241799C10) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL488-98264-6

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 488 Anti-Human Siglec-9 Rabbit RecAb (CL488-98264-6, Clone: 241799C10) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-98264-6 cible Siglec-9 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Siglec-9 Protéine recombinante Eg1084
• Nom complet: sialic acid binding Ig-like lectin 9
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: NM_014441.3
• Symbole du gène: Siglec-9
• Identification du gène (NCBI): 27180
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

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