Anticorps Recombinant de lapin anti-Siglec-E

Siglec-E Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242248H3

N° de cat : 84790-2-PBS

Synonymes

MIS, mSiglec-E, Myeloid inhibitory siglec, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin 5

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Galerie de données de validation

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  WB analysis of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84790-2-RR (same clone as 84790-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84790-2-RR (Siglec-E antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84790-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01535-1

  Cytometric bead array standard curve of MP01535-1, MOUSE Siglec-12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84790-2-PBS. Detection antibody: 84790-1-PBS. Standard: Eg2733. Range: 3.125-100 ng/mL.

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Informations sur le produit

84790-2-PBS cible Siglec-E dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Siglec-E Protéine recombinante Eg2733
• Nom complet: sialic acid binding Ig-like lectin E
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 70-90 kDa
• Numéro d’acquisition GenBank: NM_031181.2
• Symbole du gène: Siglec-E
• Identification du gène (NCBI): 83382
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).

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