Anticorps Monoclonal anti-Sortilin

Sortilin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2B11B9

N° de cat : 68007-1-PBS

Synonymes

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

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Galerie de données de validation

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  WB analysis using 68007-1-Ig (same clone as 68007-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  WB analysis using 68007-1-Ig (same clone as 68007-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68007-1-Ig (same clone as 68007-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68007-1-PBS cible Sortilin dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Sortilin Protéine recombinante Ag28043
• Nom complet: sortilin 1
• Masse moléculaire calculée: 831 aa, 92 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC023542
• Symbole du gène: Sortilin
• Identification du gène (NCBI): 6272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

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