Anticorps Polyclonal de lapin anti-Spartan

Spartan Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24850-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human placenta using 24850-1-AP (same clone as 24850-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 24850-1-AP (Spartan antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24850-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 24850-1-AP (same clone as 24850-1-PBS)

  IP result of anti-Spartan (IP:24850-1-AP, 4ug; Detection:24850-1-AP 1:500) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 24850-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24850-1-PBS cible Spartan dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Spartan Protéine recombinante Ag20586
• Nom complet: DNA-dependent metalloprotease SPRTN
• Masse moléculaire calculée: 489 aa, 55 kDa
• Poids moléculaire observé: 55 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC015740
• Symbole du gène: Spartan
• Identification du gène (NCBI): 83932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Spartan (Protein with SprT-like domain at the N terminus), also known as DVC1 or C1orf124, is a replication-coupled DNA-dependent metalloprotease that cleaves proteins cross-linked to DNA to promote DPC repair (PMID: 38600235). Spartan protease activity is stimulated by DNA binding, while posttranslational modification of Spartan governs both its protease activity and recruitment to the DPC on chromatin (PMID: 27871366). The regulation of Spartan includes a ubiquitin switch (where deubiquitination activates its proteolytic activity), and acetylation and phosphorylation switches (which facilitate DNA binding). The calculated molecular weight of Spartan is 55 kDa, while the 65-kDa band could be due to posttranslational modification (PMID: 33567341, 31316063).

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