Anticorps Polyclonal de lapin anti-Stathmin 1

• Phare
• Validé par KD/KO

Stathmin 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11157-1-PBS

Synonymes

STMN1, C1orf215, LAP18, Leukemia-associated phosphoprotein p18, OP18

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Galerie de données de validation

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  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  WB results of Stathmin antibody (11157-1-AP, 1:20000) with si-control and si-Stathmin transfected Jurkat cells.. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis using 11157-1-AP (same clone as 11157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11157-1-AP (same clone as 11157-1-PBS)

  WB result of Stathmin 1 antibody (11157-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Stathmin 1 transfected HepG2 cells. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11157-1-AP (same clone as 11157-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11157-1-AP (Stathmin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11157-1-AP (Stathmin 1 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 11157-1-AP (same clone as 11157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11157-1-AP (Stathmin 1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Stathmin 1 antibody (11157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11157-1-AP (same clone as 11157-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Stathmin 1 (11157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11157-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11157-1-PBS cible Stathmin 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Stathmin 1 Protéine recombinante Ag1633
• Nom complet: stathmin 1/oncoprotein 18
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC014353
• Symbole du gène: Stathmin 1
• Identification du gène (NCBI): 3925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.

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