Anticorps Polyclonal de lapin anti-Supervillin

Supervillin Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27524-1-PBS

Synonymes

SVIL

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Galerie de données de validation

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  WB analysis using 27524-1-AP (same clone as 27524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27524-1-AP (Supervillin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 27524-1-AP (same clone as 27524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27524-1-AP (Supervillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27524-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27524-1-PBS cible Supervillin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Supervillin Protéine recombinante Ag26196
• Nom complet: supervillin
• Masse moléculaire calculée: 248 kDa
• Poids moléculaire observé: 205 kDa
• Numéro d’acquisition GenBank: NM_003174
• Symbole du gène: SVIL
• Identification du gène (NCBI): 6840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Supervillin (SVIL) belongs to the villin/gelsolin superfamily of actin-binding proteins involved in many cellular processes. A non-muscle-specific form of Supervillin activates androgen receptor activity. Supervillin another isoform, called Archvillin, is expressed in skeletal and cardiac muscle tissue where it plays a role in both muscle fiber structure and signaling. Mutations in Supervillin are associated with myofibrillar Myopathy10 (MFM10), which causes myopathy with myofibrillar disorganization and autophagic vacuoles(PMID: 9867483, 32779703).

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