Anticorps Monoclonal anti-Synaptophysin

Synaptophysin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

4E12C4

Avis (1)

N° de cat : 67864-1-Ig

Synonymes

synaptophysin, SYP

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Galerie de données de validation

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  WB analysis using 67864-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67864-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67864-1-Ig (Synaptophysin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67864-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67864-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67864-1-Ig (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 67864-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 67864-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 67864-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Neuro-2a using 67864-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse pancreas using 67864-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (67864-1-Ig, Clone: 4E12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-12, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de lapin, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique de souris, cellules Neuro-2a, cellules PC-12, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

67864-1-Ig cible Synaptophysin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Synaptophysin Protéine recombinante Ag11803
• Nom complet: synaptophysin
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC064550
• Symbole du gène: SYP
• Identification du gène (NCBI): 6855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Publications

Species	Application	Title
mouse	IF	Front Cell Neurosci; Expression of SH3 and Multiple Ankyrin Repeat Domains Protein 3 in Mouse Retina.; Authors - Yue Xu
rat	WB	Psychiatry Res; The role of reelin in the pathological mechanism of depression from clinical to rodents; Authors - Kangyu Jin
human,mouse	WB	Alzheimers Dement; Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose; Authors - Xu-Qiao Chen
rat	IF	Neurochem Int; Photobiomodulation treatment inhibits neurotoxic astrocytic polarization and protects neurons in in vitro and in vivo stroke models; Authors - Yu Feng
mouse	WB	J Agric Food Chem; Protective Effect of 20(S)-Protopanaxadiol on D-Gal-Induced Cognitively Impaired Mice Based on Its Target Protein Brain-type Creatine Kinase; Authors - Hantao Zhang
rat	IF	BMC Complement Med Ther; A whole transcriptome profiling analysis for antidepressant mechanism of Xiaoyaosan mediated synapse loss via BDNF/trkB/PI3K signal axis in CUMS rats; Authors - Pan Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

B (Verified Customer) (01-05-2022)

Good performance in WB with a strong and clear band at 35 Kd around.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse brain lysate

Synaptophysin Antibody Western Blot validation (1:5000 dilution) in mouse brain lysate (Cat no:67864-1-Ig)