Anticorps Monoclonal anti-Synaptotagmin-1

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H1H2

N° de cat : 68043-1-PBS

Synonymes

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

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Galerie de données de validation

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  WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68043-1-PBS cible Synaptotagmin-1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Synaptotagmin-1 Protéine recombinante Ag6071
• Nom complet: synaptotagmin I
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC058917
• Symbole du gène: Synaptotagmin-1
• Identification du gène (NCBI): 6857
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

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