Anticorps Recombinant de lapin anti-Synaptotagmin-5

Synaptotagmin-5 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241452E3

N° de cat : 84452-1-PBS

Synonymes

SytV, SYT5, Synaptotagmin V, 241452E3

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Galerie de données de validation

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  WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

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  WB analysis of BxPC-3 using 84452-1-RR (same clone as 84452-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84452-1-RR (same clone as 84452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84452-1-RR (same clone as 84452-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84452-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

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Informations sur le produit

84452-1-PBS cible Synaptotagmin-5 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Synaptotagmin-5 Protéine recombinante Ag12677
• Nom complet: synaptotagmin V
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC046157
• Symbole du gène: Synaptotagmin-5
• Identification du gène (NCBI): 6861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).

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