Anticorps Recombinant de lapin anti-Synaptotagmin-5

Synaptotagmin-5 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

241452E3

N° de cat : 84452-1-RR

Synonymes

SytV, SYT5, Synaptotagmin V, 241452E3

---

Galerie de données de validation

• 

  WB analysis using 84452-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 84452-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84452-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84452-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 84452-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84452-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules BxPC-3, cellules HUVEC, cellules SW-1990, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84452-1-RR cible Synaptotagmin-5 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Synaptotagmin-5 Protéine recombinante Ag12677
• Nom complet: synaptotagmin V
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC046157
• Symbole du gène: Synaptotagmin-5
• Identification du gène (NCBI): 6861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).

{{ptg:RelatedPrimaryAntibodies}}