Anticorps Recombinant de lapin anti-CD138/Syndecan-1

CD138/Syndecan-1 Recombinant Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241278E8

N° de cat : 84069-8-RR

Synonymes

CD138, Sdc1, SYND1, Synd-1, Syndecan-1

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Galerie de données de validation

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  IHC staining of mouse spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84069-8-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84069-8-RR

  Biolayer interferometry (BLl) kinetic assays of 84069-8-RR against Mouse CD138/Syndecan-1 were performed. The affinity constant is 1.15 nM.

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu cutané de souris, tissu d'amygdalite humain, tissu de côlon de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84069-8-RR cible CD138/Syndecan-1 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD138/Syndecan-1 Protéine recombinante Eg1221
• Nom complet: syndecan 1
• Masse moléculaire calculée: 33 kDa
• Numéro d’acquisition GenBank: NM_011519.2
• Symbole du gène: Sdc1
• Identification du gène (NCBI): 20969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

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