Anticorps Monoclonal anti-Syntaxin 1B

• Phare
• Validé par KD/KO

Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D3B5

N° de cat : 66437-1-PBS

Synonymes

Syntaxin 1A / Syntaxin 1B, Syntaxin 1B, STX1B, Syntaxin 1A, 2D3B5

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Galerie de données de validation

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  WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66437-1-Ig (same clone as 66437-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig ( Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66437-1-PBS cible Syntaxin 1B dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Syntaxin 1B Protéine recombinante Ag10257
• Nom complet: syntaxin 1B
• Masse moléculaire calculée: 288 aa, 33 kDa
• Poids moléculaire observé: 33 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC062298
• Symbole du gène: STX1B
• Identification du gène (NCBI): 112755
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611). This antibody may react with both Syntaxin 1B and Syntaxin 1A.

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