Anticorps Monoclonal anti-Syntaxin 6

Syntaxin 6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B6G4

N° de cat : 60059-1-PBS

Synonymes

2B6G4, STX6, Syntaxin-6

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Galerie de données de validation

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  WB analysis using 60059-1-Ig (same clone as 60059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60059-1-Ig (same clone as 60059-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 60059-1-Ig (same clone as 60059-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 60059-1-Ig (same clone as 60059-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60059-1-Ig (Syntaxin 6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 60059-1-Ig (same clone as 60059-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (60059-1-Ig, Clone: 2B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60059-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60059-1-PBS cible Syntaxin 6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Syntaxin 6 Protéine recombinante Ag1284
• Nom complet: syntaxin 6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC009944
• Symbole du gène: Syntaxin 6
• Identification du gène (NCBI): 10228
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

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