Anticorps Monoclonal anti-TAB1

TAB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1E10D12

N° de cat : 67020-1-Ig

Synonymes

MAP3K7IP1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAK1 binding protein 1, TAK1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 67020-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67020-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67020-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67020-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67020-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67020-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67020-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

67020-1-Ig cible TAB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAB1 Protéine recombinante Ag6590
• Nom complet: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC050554
• Symbole du gène: TAB1
• Identification du gène (NCBI): 10454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Hum Mutat; The MAP3K7 gene: further delineation of clinical characteristics and genotype/phenotype correlations.; Authors - Geeske M van Woerden
human	WB,IP	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu

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