Anticorps Recombinant de lapin anti-TACC3

TACC3 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242527A12

N° de cat : 84940-5-PBS

Synonymes

242527A12, ERIC 1, ERIC1, ERIC-1, Maskin

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Galerie de données de validation

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  WB analysis using 84940-5-RR (same clone as 84940-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84940-5-RR (TACC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84940-5-RR (same clone as 84940-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84940-5-RR (TACC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84940-5-RR (same clone as 84940-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC3 antibody (84940-5-RR, Clone: 242527A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84940-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84940-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84940-5-RR against Human TACC3 were performed. The affinity constant is 2.98 nM.

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Informations sur le produit

84940-5-PBS cible TACC3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TACC3 Protéine recombinante Ag22516
• Nom complet: transforming, acidic coiled-coil containing protein 3
• Masse moléculaire calculée: 838 aa, 91 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC111771
• Symbole du gène: TACC3
• Identification du gène (NCBI): 10460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.

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