Anticorps Polyclonal de lapin anti-Neurokinin-1 receptor

Neurokinin-1 receptor Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 17942-1-AP

Synonymes

Neurokinin-1 receptor, NK 1 receptor, NK 1R, NK1R, NK-1R, NKIR, SPR, Substance P receptor, TAC1R, tachykinin receptor 1, TACR1

---

Galerie de données de validation

• 

  WB analysis using 17942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17942-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 17942-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 17942-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17942-1-AP (Neurokinin-1 receptor antibody at dilution of 1:300 incubated at 4 degree celsius over night.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules Caco-2, cellules HeLa, cellules THP-1, tissu pancréatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

17942-1-AP cible Neurokinin-1 receptor dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Neurokinin-1 receptor Protéine recombinante Ag12368
• Nom complet: tachykinin receptor 1
• Masse moléculaire calculée: 407 aa, 46 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC074911
• Symbole du gène: TACR1
• Identification du gène (NCBI): 6869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

neurokinin 1 receptor (NK1R, also known as the tachykinin 1 receptor, TACR1), a mediator of behavioral stress responses, in alcohol dependence and treatment. One such neurotransmitter is substance P (SP), which together with its preferred TACR1 is highly expressed in brain areas involved in stress responses and drug reward, including the hypothalamus, amygdala, and nucleus accumbens (PMID: 18276852).

Publications

Species	Application	Title
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
mouse	WB	PLoS One; Hemokinin-1(4-11)-Induced Analgesia Selectively Up-Regulates δ-Opioid Receptor Expression in Mice.; Authors - Cai-Yun Fu
mouse	WB	J Neuroimmunol; Tropisetron attenuates lipopolysaccharide induced neuroinflammation by inhibiting NF-κB and SP/NK1R signaling pathway.; Authors - Yan Yu
mouse	WB	Peptides; Study on the distribution sites and the molecular mechanism of analgesia after intracerebroventricular injection of rat/mouse hemokinin-1 in mice.; Authors - Xia Rui-Long RL
human	WB	Neoplasma; miR-877-5p antagonizes the promoting effect of SP on the gastric cancer progression.; Authors - Xiao-Dan Guo
human	WB	Pharm Biol; Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor; Authors - Jimei Zhang