Anticorps Recombinant de lapin anti-TADA1L

• Phare
• Validé par KD/KO

TADA1L Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240637F7

N° de cat : 83641-4-RR

Synonymes

240637F7, KIAA0764, RP1 9E21.4, SPT3 associated factor 42, SPT3-associated factor 42

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Galerie de données de validation

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  WB analysis using 83641-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 83641-4-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 83641-4-RR

  WB result of TADA1L antibody (83641-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TADA1L transfected MCF-7 cells.

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  IF Staining of MCF-7 using 83641-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 83641-4-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83641-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83641-4-RR cible TADA1L dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TADA1L Protéine recombinante Ag14118
• Nom complet: transcriptional adaptor 1 (HFI1 homolog, yeast)-like
• Masse moléculaire calculée: 335 aa, 37 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC015401
• Symbole du gène: TADA1L
• Identification du gène (NCBI): 117143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.

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