Anticorps Polyclonal de lapin anti-TAF1-Specific

TAF1-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20260-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 20260-1-AP (same clone as 20260-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20260-1-AP (same clone as 20260-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20260-1-AP (TAF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 20260-1-AP (same clone as 20260-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 20260-1-AP (TAF1-Specific antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 20260-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

20260-1-PBS cible TAF1-Specific dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• Masse moléculaire calculée: 213 kDa
• Poids moléculaire observé: 250-260 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_138923
• Symbole du gène: TAF1
• Identification du gène (NCBI): 6872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TAF1, also named as BA2R, CCG1, CCGS, TAF2A, p250, Cell cycle gene 1 protein, TAFII250 and DYT3, belongs to the TAF1 family. TAF1 is the largest component and core scaffold of the TFIID basal transcription factor complex. It contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. TAF1 phosphorylates GTF2A1 and GTF2F1 on Ser residues. It possesses DNA-binding activity. It is essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3) which also called X-linked dystonia-parkinsonism (XDP). The antibody is specific to TAF1. It has several isoforms with predicted MW's of 200-250 kDa and 170-180 kDa, it also could get about 50 kDa band which is caused by the lysate preparation.

{{ptg:RelatedPrimaryAntibodies}}