Anticorps Polyclonal de lapin anti-TAF15

• Phare
• Validé par KD/KO

TAF15 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 25521-1-AP

Synonymes

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

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Galerie de données de validation

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  WB analysis of Jurkat using 25521-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25521-1-AP

  WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

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  WB analysis of mouse brain using 25521-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25521-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25521-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25521-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25521-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25521-1-AP (TAF15 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules HEK-293, cellules HeLa, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain, tissu de mélanome malin humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

25521-1-AP cible TAF15 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: NM_003487
• Symbole du gène: TAF15
• Identification du gène (NCBI): 8148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Biol Chem; TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells; Authors - Rukiye Nar
human	WB,IP,IF	F1000Res; A guide to selecting high-performing antibodies for TAF15 (UniProt ID: Q92804) for use in western blot, immunoprecipitation, and immunofluorescence; Authors - Vera Ruíz Moleón ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manohar (Verified Customer) (05-13-2020)

There are multiple bands appearing on western blot. When used with 5% blocking milk and 1:1000 antibody dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

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