Anticorps Polyclonal de lapin anti-TAF9

• Phare
• Validé par KD/KO

TAF9 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 10544-1-AP

Synonymes

AD 004, AK6, CGI 137, CINAP, CIP, STAF31/32, TAF2G, TAF9, TAFII 31, TAFII 32, TAFII31, TAFII32, TAFIID32

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Galerie de données de validation

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  WB analysis of HeLa using 10544-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10544-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10544-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10544-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10544-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 10544-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10544-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10544-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10544-1-AP (TAF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 10544-1-AP

  IP result of anti-TAF9 (IP:10544-1-AP, 4ug; Detection:10544-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, tissu cardiaque humain, tissu de côlon humain, tissu de muscle squelettique humain, tissu hépatique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10544-1-AP cible TAF9 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAF9 Protéine recombinante Ag0833
• Nom complet: TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC007426
• Symbole du gène: TAF9
• Identification du gène (NCBI): 6880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAF9, also named as TAF2G, TAFII31, is a 264 amino acid protein, which belongs to the TAF9 family. TAF9 are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. TAF9 may have a role in gene regulation associated with apoptosis.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	WB	Front Oncol; Stable Isotope Tracing Uncovers Reduced γ/β-ATP Turnover and Metabolic Flux Through Mitochondrial-Linked Phosphotransfer Circuits in Aggressive Breast Cancer Cells.; Authors - Aleksandr Klepinin
Human	WB	J Biol Chem; The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites.; Authors - Fumitoshi Irie
mouse,rat	WB,IHC,IP	Front Pharmacol; Activation of TAF9 via Danshensu-Induced Upregulation of HDAC1 Expression Alleviates Non-alcoholic Fatty Liver Disease.; Authors - Ruiwen Wang ; KD Validated
human	WB	J Proteome Res; Targeted Proteomic Analysis Revealed Kinome Reprogramming during Acquisition of Radioresistance in Breast Cancer Cells.; Authors - Weili Miao
human	WB	Mol Cell Proteomics; Multi-Level Proteomics Reveals Epigenetic Signatures in BCG-mediated Macrophage Activation; Authors - Zoe Schaefer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (10-08-2024)

good, very low on blot - convenient

• Applications: Western Blot

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