Anticorps Recombinant de lapin anti-transgelin/SM22

transgelin/SM22 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240864A11

N° de cat : 83922-2-PBS

Synonymes

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

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Galerie de données de validation

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  WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83922-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

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Informations sur le produit

83922-2-PBS cible transgelin/SM22 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: transgelin/SM22 Protéine recombinante Ag0764
• Nom complet: transgelin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC004927
• Symbole du gène: transgelin/SM22
• Identification du gène (NCBI): 6876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.

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