Anticorps Polyclonal de lapin anti-TAGLN3

TAGLN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 12246-1-AP

Synonymes

Neuronal protein 22, Neuronal protein NP25, TAGLN3, transgelin 3

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Galerie de données de validation

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  WB analysis of mouse brain using 12246-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12246-1-AP (TAGLN3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12246-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12246-1-AP (TAGLN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12246-1-AP

  IP result of anti-TAGLN3 (IP:12246-1-AP, 4ug; Detection:12246-1-AP 1:2000) with mouse brain tissue lysate 3160 ug.

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  IHC staining of human brain using 12246-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12246-1-AP (TAGLN3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 12246-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12246-1-AP (TAGLN3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

12246-1-AP cible TAGLN3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAGLN3 Protéine recombinante Ag2891
• Nom complet: transgelin 3
• Masse moléculaire calculée: 22.4 kDa
• Poids moléculaire observé: 22.4 kDa
• Numéro d’acquisition GenBank: BC015329
• Symbole du gène: TAGLN3
• Identification du gène (NCBI): 29114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgenlin 3 protein. It may cross-react with other two transgenlins based on the sequence similarity.

Publications

Species	Application	Title
mouse	IHC	Sci Rep; microRNA regulatory circuits in a mouse model of inherited retinal degeneration.; Authors - Arpad Palfi

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