Anticorps Monoclonal anti-TAK1

TAK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1A5B2

N° de cat : 67707-1-PBS

Synonymes

MAP3K7, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7, EC:2.7.11.25

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Galerie de données de validation

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  WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67707-1-Ig (same clone as 67707-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67707-1-Ig (same clone as 67707-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 μg TAK1 Monoclonal antibody (67707-1-Ig, Clone:1A5B2 (red) or Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3 (blue), and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67707-1-PBS cible TAK1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAK1 Protéine recombinante Ag21286
• Nom complet: mitogen-activated protein kinase kinase kinase 7
• Masse moléculaire calculée: 579 aa, 64 kDa
• Poids moléculaire observé: 67-70 kDa
• Numéro d’acquisition GenBank: BC017715
• Symbole du gène: TAK1
• Identification du gène (NCBI): 6885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

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