Anticorps Polyclonal de lapin anti-TANC2

TANC2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 31541-1-AP

Synonymes

ROLSA, rols, KIAA1636, KIAA1148

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Galerie de données de validation

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  WB analysis using 31541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31541-1-AP (TANC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 31541-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TANC2 antibody (31541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31541-1-AP cible TANC2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TANC2 Protéine recombinante Ag35427
• Nom complet: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
• Masse moléculaire calculée: 219kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: NM_001394998
• Symbole du gène: TANC2
• Identification du gène (NCBI): 26115
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 2 (TANC2), also known as KIAA1148 and KIAA1636, belongs to the TANC family. TANC2 is a large 220 kDa protein consisting of 1990 amino acid residues. Named after its domain architecture, it comprises tetratricopeptide (TPR) and ankyrin repeats (ANK), a coiled-coil domain, and a C-terminal PDZ-interacting motif. TANC2 is widely expressed in the developing human (in excitatory neurons and radial glial cells) and rodent brain as well as in the adult brain as a scaffold in the postsynaptic density (PSD) of excitatory neurons. As such, it facilitates cell signaling downstream of cell surface glutamate receptors through multi-protein complex formation with other PSD proteins (e.g. PSD95, SHANK1), influencing dendritic spine formation and synaptic strength (PMID: 33976205, 34964047).

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