Anticorps Polyclonal de lapin anti-TAOK3

TAOK3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10158-2-AP

Synonymes

DPK, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

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Galerie de données de validation

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  WB analysis of human liver using 10158-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10158-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10158-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10158-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10158-2-AP

  IP Result of anti-TAOK3 (IP:10158-2-AP, 4ug; Detection:10158-2-AP 1:700) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HeLa, cellules HepG2, tissu cérébral humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10158-2-AP cible TAOK3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAOK3 Protéine recombinante Ag0211
• Nom complet: TAO kinase 3
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC002756
• Symbole du gène: TAOK3
• Identification du gène (NCBI): 51347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Publications

Species	Application	Title
human	WB	Cell Commun Signal; Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.; Authors - Tsung-Ching Lai ; KD Validated
mouse	IP	Sci Signal; The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation; Authors - Alexandre Poirier