Anticorps Polyclonal de lapin anti-TAP1

• Phare
• Validé par KD/KO

TAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(32)

Avis (1)

N° de cat : 11114-1-AP

Synonymes

RING4, PSF-1, PSF1, EC:7.4.2.14, ATP-binding cassette sub-family B member 2

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Galerie de données de validation

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  WB analysis using 11114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11114-1-AP

  WB result of TAP1 antibody (11114-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAP1 transfected HeLa cells.

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  WB analysis using 11114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11114-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11114-1-AP (TAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11114-1-AP

  IP result of anti-TAP1 (IP:11114-1-AP, 4ug; Detection:11114-1-AP 1:3000) with HepG2 cells lysate 1320 ug.

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  IHC staining of human pancreas cancer using 11114-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 11114-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11114-1-AP (TAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 11114-1-AP

  Immunofluorescent analysis of HepG2 cells, using TAP1 antibody 11114-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 11114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11114-1-AP (TAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HeLa, cellules HepG2, cellules SW480, tissu de muscle squelettique de souris, tissu splénique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 13 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

11114-1-AP cible TAP1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAP1 Protéine recombinante Ag1619
• Nom complet: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 70-81 kDa
• Numéro d’acquisition GenBank: BC014081
• Symbole du gène: TAP1
• Identification du gène (NCBI): 6890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.

Publications

Species	Application	Title
human	WB	Gastroenterology; microRNA 125a Regulates MHC-I Expression on Esophageal Adenocarcinoma Cells, Associated With Suppression of Antitumor Immune Response and Poor Outcomes of Patients.; Authors - Luigi Mari
human	IHC	Nat Commun; Proteomic analysis of archival breast cancer clinical specimens identifies biological subtypes with distinct survival outcomes.; Authors - Karama Asleh
human	WB,IP	Proc Natl Acad Sci U S A; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
mouse	WB	Theranostics; Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.; Authors - Jingyao Tu
human,mouse	WB	Int J Cancer; Antitumor efficacy of intermittent low-dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche; Authors - Chakrapani Tripathi
human	IHC	Int J Mol Sci; Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers.; Authors - Boya Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adrianna (Verified Customer) (10-24-2022)

This antibody is excellent. I've tried anti TAP1 antibodies from different companies and they all showed brighter bands for contaminants than the TAP1 protein, here I can clearly see my protein of interest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

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