Anticorps Monoclonal anti-TAP1

TAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B8A7

N° de cat : 68412-1-PBS

Synonymes

ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3

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Galerie de données de validation

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  WB analysis using 68412-1-Ig (same clone as 68412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68412-1-Ig (TAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TAP1 antibody (68412-1-Ig, Clone: 1B8A7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68412-1-PBS cible TAP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAP1 Protéine recombinante Ag21303
• Nom complet: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC014081
• Symbole du gène: TAP1
• Identification du gène (NCBI): 6890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.

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