Anticorps Polyclonal de lapin anti-TDP-43 (full length)

TDP-43 (full length) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 18280-1-AP

Synonymes

TARDBP, TDP 43, TDP43, TDP-43, ALS10

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Galerie de données de validation

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  WB analysis using 18280-1-AP

  K562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 18280-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 18280-1-AP

  IP result of anti-TDP-43 (IP:18280-1-AP, 3ug; Detection:18280-1-AP 1:1500) with K-562 cells lysate 6000ug.

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  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18280-1-AP (TDP-43 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 18280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18280-1-AP (TDP-43 (full length) antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 18280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (18280-1-AP) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• 

  FC experiment of SH-SY5Y using 18280-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug TDP-43 (full length) Polyclonal antibody (18280-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1500-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

18280-1-AP cible TDP-43 (full length) dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001487
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Publications

Species	Application	Title
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière
human	IHC	Acta Neuropathol Commun; Anatomic survey of seeding in Alzheimer's disease brains reveals unexpected patterns.; Authors - Barbara E Stopschinski
human	WB	Neurobiol Dis; Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology; Authors - Yuan Tian
mouse	IF	Dis Model Mech; Viral delivery of C9ORF72 hexanucleotide repeat expansions in mice lead to repeat length dependent neuropathology and behavioral deficits.; Authors - Saul Herranz-Martin
human	IHC	J Neurotrauma; Multimodal Biomarkers of Repetitive Head Impacts and Traumatic Encephalopathy Syndrome: A Clinico-Pathological Case Series.; Authors - Breton Asken
human	IHC	J Alzheimers Dis; Pathological Correlates of Cognitive Impairment in The University of Manchester Longitudinal Study of Cognition in Normal Healthy Old Age.; Authors - Andrew C Robinson

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