Anticorps Monoclonal anti-TDP-43 (human specific)

• Phare
• Validé par KD/KO

TDP-43 (human specific) Monoclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

98

CloneNo.

6H6E12

Avis (2)

N° de cat : 60019-2-Ig

Synonymes

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43, TDP-43 (human specific)

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Galerie de données de validation

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  WB analysis using 60019-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 60019-2-Ig

  IP Result of anti-TDP-43 (human specific) (IP:60019-2-Ig, 5ug; Detection:60019-2-Ig 1:1000) with K-562 cells lysate 1720ug.

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  IHC staining of human gliomas using 60019-2-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain(FTLD-U) tissue using 60019-2-Ig

  40X of FTLD-U case stained by 10782-2-AP and 60019-2-Ig, showing dystrophic neurites. (Figs were provided by Linda K. Kwong).

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  IHC staining of human pancreas cancer using 60019-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 60019-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60019-2-Ig (TDP-43 (human specific) antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 60019-2-Ig

  1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 (human specific) antibody (60019-2-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain (DLFT-U), tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 52 publications below
• IHC: See 26 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

60019-2-Ig cible TDP-43 (human specific) dans les applications de WB, IP, IHC, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, cellule de levure
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001487
• Symbole du gène: TARDBP
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.

Publications

Species	Application	Title
human	WB	Nature; Amyloid fibrils in disease FTLD-TDP are composed of TMEM106B not TDP-43.; Authors - Yi Xiao Jiang
	WB	Nature; A single-cell atlas of human and mouse white adipose tissue.; Authors - Margo P Emont
human	IHC	Nature; Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.; Authors - Deng Han-Xiang HX
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB,IP,IHC	Nat Neurosci; TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.; Authors - Manling Xie
		Nat Neurosci; TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.; Authors - Florent Laferrière

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lidiya (Verified Customer) (05-26-2022)

The antibody works great! Antibody was used for WB in Neuroblastoma cell lines (SK-N-BE(2)C and IMR32) with HRP-conjugated secondary. Antibody was also used for a Proximity Ligation Assay to assess interaction with another protein in SK-N-BE(2)C cells. 1:50 of antibody was used for this assay.

• Applications: Western Blot, Other
• Primary Antibody Dilution: 1:2000 (WB)
• Cell Tissue Type: Neuroblastoma Cell Lines

TDP-43 (human specific) Antibody Western Blot,Other validation (1:2000 (WB) dilution) in Neuroblastoma Cell Lines (Cat no:60019-2-Ig)

FH

David (Verified Customer) (01-13-2020)

Great antibody for several applications. Works nicely for immunoblot and immunofluourescence. Can also be used for RNA immunoprecipitation.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:500 (IF); 1:1000 (WB); 30µg (RIP)
• Cell Tissue Type: SH-SY5Y

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