Anticorps Monoclonal anti-TARS

TARS Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1A11F10

N° de cat : 67828-1-Ig

Synonymes

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

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Galerie de données de validation

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  WB analysis using 67828-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HSC-T6 using 67828-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67828-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 67828-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67828-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules PC-12
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67828-1-Ig cible TARS dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TARS Protéine recombinante Ag6614
• Nom complet: threonyl-tRNA synthetase
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 83-87 kDa
• Numéro d’acquisition GenBank: BC000517
• Symbole du gène: TARS
• Identification du gène (NCBI): 6897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Publications

Species	Application	Title
human	WB	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu

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