Anticorps Polyclonal de lapin anti-TASOR

TASOR Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (3)

N° de cat : 30188-1-AP

Synonymes

C3orf63, FAM208A, se89 1, RAP140, KIAA1105

---

Galerie de données de validation

• 

  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30188-1-AP cible TASOR dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TASOR Protéine recombinante Ag32757
• Nom complet: chromosome 3 open reading frame 63
• Masse moléculaire calculée: 189 kDa
• Poids moléculaire observé: 189 kDa
• Numéro d’acquisition GenBank: NM_001112736.1
• Symbole du gène: TASOR
• Identification du gène (NCBI): 23272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TASOR, also known as FAM208A or C3orf63, is the central subunit of the human silencing hub (HUSH) complex. HUSH regulates deposition of the epigenetic mark H3K9me3. TASOR has a catalytically-inactive PARP domain which is necessary for targeted H3K9me3 deposition (PMID: 33009411). TASOR and the RNA deadenylase CCR4-NOT complex scaffold CNOT1 synergistically repress HIV proviral expression (PMID: 35013187). TASOR affects the genome stability during the cellular division and also interacts with methylation complex in adult tissues (PMID: 34272044).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tsimafei (Verified Customer) (09-22-2025)

Antibody recognises the band of the expected size as well as several non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: U2OS

TASOR Antibody Western Blot validation (1:1500 dilution) in U2OS (Cat no:30188-1-AP)

FH

Julia (Verified Customer) (07-02-2025)

Works well! Validated with KO.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESC

TASOR Antibody Western Blot validation (1:1000 dilution) in mESC (Cat no:30188-1-AP)

FH

Roy (Verified Customer) (06-12-2024)

Could detect TASOR in HeLa cells and Jurkat cells (1/1000 dilution - Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa, Jurkat

{{ptg:RelatedPrimaryAntibodies}}