Anticorps Polyclonal de lapin anti-TAU

TAU Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

N° de cat : 10274-1-PBS

Synonymes

MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC

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Galerie de données de validation

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  WB analysis of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10274-1-AP (TAU antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10274-1-AP (TAU Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10274-1-AP (same clone as 10274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10274-1-AP (TAU antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 10274-1-AP (same clone as 10274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using TAU antibody (10274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10274-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10274-1-PBS cible TAU dans les applications de WB, IHC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAU Protéine recombinante Ag0354
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 45-70 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

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