Anticorps Monoclonal anti-TAU

• Phare
• Validé par KD/KO

TAU Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2c

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E9A8

N° de cat : 66499-1-PBS

Synonymes

MAPT, 1E9A8, AT180, DDPAC, MAPTL

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Galerie de données de validation

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  WB analysis of HeLa using 66499-1-Ig (same clone as 66499-1-PBS)

  WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66499-1-PBS cible TAU dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2c
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TAU Protéine recombinante Ag21926
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.

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