Anticorps Polyclonal de lapin anti-TAX1BP3

TAX1BP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (4)

N° de cat : 11692-1-AP

Synonymes

Tax interaction protein 1, Tax1 binding protein 3, TAX1BP3, TIP 1, TIP1

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Galerie de données de validation

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  WB analysis using 11692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11692-1-AP (TAX1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11692-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11692-1-AP (TAX1BP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

11692-1-AP cible TAX1BP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAX1BP3 Protéine recombinante Ag2306
• Nom complet: Tax1 (human T-cell leukemia virus type I) binding protein 3
• Masse moléculaire calculée: 124 aa, 14 kDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: BC023980
• Symbole du gène: TAX1BP3
• Identification du gène (NCBI): 30851
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tax-interacting protein 1 (TIP-1, also known as Tax1bp3 or glutaminase-interacting protein, GIP) is unique in its simple structure, a single PDZ domain is the only functional and structural unit identified so far in this small PDZ protein (124 amino acids in human and mouse). High level of TIP-1 expression was detected in human invasive breast cancer cells and that is important for tumor cell adhesion, migration and pulmonary metastasis. However, the precise biological effects of TIP-1 and its functional mechanisms have largely remained elusive. Mutation of TAX1BP3 can cause Dilated Cardiomyopathy with Septo-Optic Dysplasia (PMID: 25645515).

Publications

Species	Application	Title
human,mouse	WB,IHC	Gene; Birc3 and Tip1 are upregulated in renal ischemia reperfusion injury; Authors - Sixu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Good clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-23-2020)

works well with a specific band that matches the product size

• Applications: Western Blot,
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Human kidney biopsy

FH

Canzhao (Verified Customer) (01-17-2020)

It is one of the best antibody I used. It is very specific. I would like to give it 6 stars if it is possible.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
• Cell Tissue Type: Mouse heart and cultured cardiomyocytes.

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