Anticorps Monoclonal anti-WWTR1/TAZ

• Phare
• Validé par KD/KO

WWTR1/TAZ Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A12A10

N° de cat : CL488-66500

Synonymes

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, 2A12A10

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Galerie de données de validation

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  WB analysis using CL488-66500

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66500 (WWTR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using CL488-66500

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66500 (WWTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MDA-MB-231

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66500 cible WWTR1/TAZ dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: WWTR1/TAZ Protéine recombinante Ag13330
• Nom complet: WW domain containing transcription regulator 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014052
• Symbole du gène: WWTR1
• Identification du gène (NCBI): 25937
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

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