Anticorps Monoclonal anti-TBC1D25

TBC1D25 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C7H3

N° de cat : 67574-1-PBS

Synonymes

2C7H3, TBC1 domain family member 25, TBC1 domain family, member 25

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Galerie de données de validation

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  WB analysis using 67574-1-Ig (same clone as 67574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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  WB analysis using 67574-1-Ig (same clone as 67574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67574-1-Ig (same clone as 67574-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 67574-1-Ig (same clone as 67574-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67574-1-PBS cible TBC1D25 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBC1D25 Protéine recombinante Ag29470
• Nom complet: TBC1 domain family, member 25
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC101817
• Symbole du gène: TBC1D25
• Identification du gène (NCBI): 4943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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