Anticorps Polyclonal de lapin anti-TBC1D5

• Phare
• Validé par KD/KO

TBC1D5 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (3)

N° de cat : 17078-1-AP

Synonymes

KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5, TBC1D5

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Galerie de données de validation

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  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 17078-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 17078-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 17078-1-AP (TBC1D5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17078-1-AP

  IP Result of anti-TBC1D5 (IP:17078-1-AP, 4ug; Detection:17078-1-AP 1:300) with A431 cells lysate 2400ug.

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  IHC staining of mouse testis using 17078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17078-1-AP (TBC1D5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17078-1-AP (TBC1D5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules A431, cellules Jurkat, cellules PC-3
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

17078-1-AP cible TBC1D5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBC1D5 Protéine recombinante Ag10991
• Nom complet: TBC1 domain family, member 5
• Masse moléculaire calculée: 795 aa, 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: BC013145
• Symbole du gène: TBC1D5
• Identification du gène (NCBI): 9779
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.

Publications

Species	Application	Title
human	WB,IF	Sci Adv; De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex.; Authors - Kai-En Chen
human	WB	EMBO J; Control of RAB7 activity and localization through the retromer-TBC1D5 complex enables RAB7-dependent mitophagy.; Authors - Ana Jimenez-Orgaz ; KO Validated
human	WB	J Cell Biol; Retromer and TBC1D5 maintain late endosomal RAB7 domains to enable amino acid-induced mTORC1 signaling.; Authors - Arunas Kvainickas ; KO Validated
	WB	EMBO Rep; BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.; Authors - Ashari R Kannangara
human	WB	Int J Biol Sci; TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression.; Authors - Diyuan Zhou ; KD Validated
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

q (Verified Customer) (06-01-2021)

It is OK to detect TBC1D5 (100 kD) in both mouse and postmortem brain lysates, although there are some non specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain and postmortembrain lysates

TBC1D5 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:17078-1-AP)

FH

Florian (Verified Customer) (04-09-2019)

This is a very strong antibody that is reasonably specific in western blot applications. We have validated it through Crispr/Cas9 mediated knockout of TBC1D5 in HeLa cells (Jimenez-Orgaz et al, EMBOJ, 2018). While the strongest band is indeed TBC1D5, as our knockout confirms, it also produces several weaker, unspecific bands on membranes blocked with 5% milk. Because of the unspecific bands, I rate it with four stars instead of five. We have not tested it for immunofluorescence. Overall, it is a very good antibody that will probably also work at much lower dilutions than 1:1000. Diluting it further may also reduce the unspecific signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela cells

FH

David (Verified Customer) (02-28-2019)

Hela cells stained with anti-TBC1D5 (green) and Hoechst (blue)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Hela cells

TBC1D5 Antibody Western Blot,Immunofluorescence, validation (1:200 dilution) in Hela cells (Cat no:17078-1-AP)