Anticorps Monoclonal anti-TBCC

TBCC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B7D9

N° de cat : 68371-1-PBS

Synonymes

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9

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Galerie de données de validation

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  WB analysis using 68371-1-Ig (same clone as 68371-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

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  WB analysis of Calu-3 using 68371-1-Ig (same clone as 68371-1-PBS)

  Calu-3 cells were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68371-1-Ig (same clone as 68371-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBCC antibody (68371-1-Ig, Clone: 2B7D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68371-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68371-1-PBS cible TBCC dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBCC Protéine recombinante Ag8962
• Nom complet: tubulin folding cofactor C
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC020170
• Symbole du gène: TBCC
• Identification du gène (NCBI): 6903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).

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