Anticorps Polyclonal de lapin anti-TBCD

TBCD Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14867-1-AP

Synonymes

Beta tubulin cofactor D, KIAA0988, SSD 1, SSD1, TBCD, tfcD, tubulin folding cofactor D, Tubulin specific chaperone D

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Galerie de données de validation

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  WB analysis using 14867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14867-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of mouse brain using 14867-1-AP

  IP Result of anti-TBCD (IP:14867-1-AP, 4ug; Detection:14867-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14867-1-AP cible TBCD dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBCD Protéine recombinante Ag6310
• Nom complet: tubulin folding cofactor D
• Masse moléculaire calculée: 133 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC003094
• Symbole du gène: TBCD
• Identification du gène (NCBI): 6904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	ACS Chem Neurosci; Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity; Authors - Xinglai Zhang
human	IF	Genes Chromosomes Cancer; MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines.; Authors - Alexandra Kuzyk
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens
human	WB	Front Genet; Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.; Authors - Hongxia Yuan