Anticorps Recombinant de lapin anti-TBCD

• Phare
• Validé par KD/KO

TBCD Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230209H7

N° de cat : 82911-1-PBS

Synonymes

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

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Galerie de données de validation

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  WB analysis using 82911-1-RR (same clone as 82911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

  WB result of TBCD antibody (82911-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBCD transfected MCF-7 cells. This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 82911-1-RR (same clone as 82911-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82911-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82911-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

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Informations sur le produit

82911-1-PBS cible TBCD dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TBCD Protéine recombinante Ag6310
• Nom complet: tubulin folding cofactor D
• Masse moléculaire calculée: 133 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC003094
• Symbole du gène: TBCD
• Identification du gène (NCBI): 6904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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