Anticorps Monoclonal anti-TBK1

• Phare
• Validé par KD/KO

TBK1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

2D7B1

N° de cat : 67211-1-Ig

Synonymes

FLJ11330, NAK, NF kappa B activating kinase, T2K, TANK binding kinase 1, TBK1

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Galerie de données de validation

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  WB analysis using 67211-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67211-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 67211-1-Ig

  U2OS (WT and TBK1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 67211-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 67211-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

67211-1-Ig cible TBK1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBK1 Protéine recombinante Ag28894
• Nom complet: TANK-binding kinase 1
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC034950
• Symbole du gène: TBK1
• Identification du gène (NCBI): 29110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

Publications

Species	Application	Title
mouse	WB	J Med Chem; Development of Orally Bioavailable Amidobenzimidazole Analogues Targeting Stimulator of Interferon Gene (STING) Receptor; Authors - Nan-Nan Chen
human	WB	Front Immunol; Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.; Authors - Zhihai Zhou
human	WB	Heliyon; Metformin promotes cGAS/STING signaling pathway activation by blocking AKT phosphorylation in gastric cancer; Authors - Qian Shen
human	WB	Biomed Pharmacother; IFI16 promotes cervical cancer progression by upregulating PD-L1 in immunomicroenvironment through STING-TBK1-NF-kB pathway.; Authors - Hongning Cai
human	WB	F1000Res; Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence; Authors - Walaa Alshafie
human	WB	Int J Mol Sci; Functional Characterization of a Familial ALS-Associated Missense TBK1 (p-Arg573Gly) Mutation in Patient-Derived Lymphoblasts; Authors - Gracia Porras