Anticorps Recombinant de lapin anti-TBK1

TBK1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240769G5

N° de cat : 83686-3-PBS

Synonymes

240769G5, EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1

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Galerie de données de validation

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  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83686-3-RR (same clone as 83686-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (83686-3-RR, Clone: 240769G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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Informations sur le produit

83686-3-PBS cible TBK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TBK1 Protéine recombinante Ag28894
• Nom complet: TANK-binding kinase 1
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC034950
• Symbole du gène: TBK1
• Identification du gène (NCBI): 29110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

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