Anticorps Monoclonal anti-TBP

TBP Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3B2

N° de cat : CL488-66166

Synonymes

TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1

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Galerie de données de validation

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CL488-66166 (TBP antibody) at dilution of 1:50.

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  IF Staining of A431 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488-conjugated TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66166

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human TBP (CL488-66166, Clone:2H3B2) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules C2C12, cellules A431
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66166 cible TBP dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBP Protéine recombinante Ag12383
• Nom complet: TATA box binding protein
• Masse moléculaire calculée: 338 aa, 38 kDa
• Numéro d’acquisition GenBank: BC110341
• Symbole du gène: TBP
• Identification du gène (NCBI): 6908
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

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