Anticorps Polyclonal de lapin anti-TBPL1

TBPL1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 12258-1-AP

Synonymes

21 kDa TBP like protein, STUD, TBP like 1, TBP like factor, TBP like protein 1, TBP related factor 2, TBP related protein, TBPL1, TLF, TLP, TLP21, TRF2, TRP

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Galerie de données de validation

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  WB analysis of mouse testis using 12258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12258-1-AP (TBPL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12258-1-AP

  IP result of anti-TBPL1 (IP:12258-1-AP, 4ug; Detection:12258-1-AP 1:300) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris,
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu d'estomac de souris, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12258-1-AP cible TBPL1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBPL1 Protéine recombinante Ag2880
• Nom complet: TBP-like 1
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 21 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC017559
• Symbole du gène: TBPL1
• Identification du gène (NCBI): 9519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Cell; A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.; Authors - Peng Dai
mouse	WB	Pharmacol Res; Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway; Authors - Xiaohua Xie
human	WB	Cell Signal; C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1.; Authors - Hui Qin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jianing (Verified Customer) (09-03-2019)

This antibody recognize human tbpl1 strongly which give a nice clear band. But the efficiency against zebrafish tbpl1 is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell culture lysate