Anticorps Monoclonal anti-TBR1

TBR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

5C3A5

N° de cat : 66564-1-PBS

Synonymes

T box brain protein 1, T box, brain, 1, T brain 1, T-box brain protein 1, TBR 1

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Galerie de données de validation

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  WB analysis using 66564-1-Ig (same clone as 66564-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  WB analysis of pig cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66564-1-Ig (same clone as 66564-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66564-1-Ig (same clone as 66564-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66564-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66564-1-PBS cible TBR1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TBR1 Protéine recombinante Ag15262
• Nom complet: T-box, brain, 1
• Masse moléculaire calculée: 682 aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC104844
• Symbole du gène: TBR1
• Identification du gène (NCBI): 10716
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

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