Anticorps Recombinant de lapin anti-TBX20

TBX20 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240385H6

N° de cat : 83414-3-PBS

Synonymes

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

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Galerie de données de validation

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  IHC staining of mouse heart using 83414-3-RR (same clone as 83414-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83414-3-RR (TBX20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 83414-3-RR (same clone as 83414-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TBX20 antibody (83414-3-RR, Clone: 240385H6 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 83414-3-RR (same clone as 83414-3-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-3-RR, Clone:240385H6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00401-1

  Cytometric bead array standard curve of MP00401-1, TBX20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83414-3-PBS. Detection antibody: 83414-1-PBS. Standard: Ag18501. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83414-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83414-3-RR against Human TBX20 were performed. The affinity constant is 17 nM.

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Informations sur le produit

83414-3-PBS cible TBX20 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TBX20 Protéine recombinante Ag18501
• Nom complet: T-box 20
• Masse moléculaire calculée: 297 aa, 33 kDa
• Numéro d’acquisition GenBank: BC120946
• Symbole du gène: TBX20
• Identification du gène (NCBI): 57057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.

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