Anticorps Polyclonal de lapin anti-TBX3

TBX3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 16741-1-AP

Synonymes

T box 3, T box protein 3, TBX3, TBX3 ISO, UMS, XHL

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Galerie de données de validation

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  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16741-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16741-1-AP (TBX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 16741-1-AP

  IP Result of anti-TBX3 (IP:16741-1-AP, 4ug; Detection:16741-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16741-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16741-1-AP (TBX3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 4 publications below

Informations sur le produit

16741-1-AP cible TBX3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBX3 Protéine recombinante Ag9955
• Nom complet: T-box 3
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC025258
• Symbole du gène: TBX3
• Identification du gène (NCBI): 6926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBX3, also named as T-box protein 3, is a 743 amino acid protein, which contains 1 T-box DNA-binding domain. TBX3 may localize in the nucleus and is widely expressed in various tissues. TBX3 as a transcriptional repressor is involved in developmental processes and probably plays a role in limb pattern formation. TBX3 plays an important role on osteogenic differentiation and proliferation of human mesenchymal stem cell derived from adipose tissue.

Publications

Species	Application	Title
	IHC	Nat Commun; Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors.; Authors - Peitao Zhang
marmoset	WB	Int J Mol Med; Leukemia inhibitory factor regulates marmoset induced pluripotent stem cell proliferation via a PI3K/‌Akt‑dependent Tbx‑3 activation pathway.; Authors - Minxia Ke
human	WB,IHC	Cell Signal; A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells.; Authors - Du Hong Fei HF
human	IHC	Hum Genomics; Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing.; Authors - Huilin Xie
human	WB,IHC	Exp Cell Res; HOTAIR maintains the stemness of ovarian cancer stem cells via the miR-206/TBX3 axis.; Authors - Yifan Zhang
	WB	Genesis; Homologous recombination efficiency enhanced by inhibition of MEK and GSK3β; Authors - Zhaoyu Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Li (Verified Customer) (12-03-2018)

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human cell lines and mouse liver tissue

TBX3 Antibody Western Blot,Immunohistochemistry, validation (1:1000 dilution) in human cell lines and mouse liver tissue (Cat no:16741-1-AP)