Anticorps Polyclonal de lapin anti-TBXA2R

• Phare
• Validé par KD/KO

TBXA2R Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27159-1-AP

Synonymes

Prostanoid TP receptor, TBXA2R, thromboxane A2 receptor, TP, TXA2 R

---

Galerie de données de validation

• 

  WB analysis using 27159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27159-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 27159-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27159-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 27159-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27159-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules DU 145, cellules HeLa, cellules MCF-7, cellules PC-3, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

27159-1-AP cible TBXA2R dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TBXA2R Protéine recombinante Ag25670
• Nom complet: thromboxane A2 receptor
• Masse moléculaire calculée: 343aa,37 kDa; 1043aa,113 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC074750
• Symbole du gène: TBXA2R
• Identification du gène (NCBI): 6915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TBXA2R, also named as thromboxane A2 receptor, TXA2-R and Prostanoid TP receptor, belongs to G-protein coupled receptor 1 family. Thromboxane A2 receptor is a key molecule in hemostasis as its abnormality leads to bleeding disorders. The TXA2-R receptor is linked via a guanine nucleotide-binding protein (G protein) to phospholipase C (PLC), which hydrolyses phosphoinositides to the two potent stimulatory second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol. IP3 causes increases in cytoplasmic free calcium and diacylglycerol causes activation of protein kinase C. In the kidney, the binding of TXA2-R to glomerular TP receptors causes intense vasoconstriction. The two isoforms expressed in cultured cells show similar ligand binding characteristics and phospholipase C (PLC) activation but oppositely regulate adenylyl cyclase activity (PMID: 8613548).

Publications

Species	Application	Title
mouse	WB	Am J Respir Crit Care Med; Thromboxane-Prostanoid Receptor Signaling Drives Persistent Fibroblast Activation in Pulmonary Fibrosis; Authors - Toshio Suzuki ; KO Validated
rat	WB, IHC	Front Endocrinol (Lausanne); The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.; Authors - Meihe Li