Anticorps Polyclonal de lapin anti-TCEB1

TCEB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12450-1-AP

Synonymes

SIII p15, Elongin-C, Elongin C, Elongin 15 kDa subunit, EloC

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Galerie de données de validation

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  WB analysis using 12450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12450-1-AP (TCEB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12450-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12450-1-AP (TCEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12450-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCEB1 antibody (12450-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12450-1-AP cible TCEB1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCEB1 Protéine recombinante Ag3087
• Nom complet: transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• Masse moléculaire calculée: 112 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC013809
• Symbole du gène: TCEB1
• Identification du gène (NCBI): 6921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
human	WB	Cell Death Differ; CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.; Authors - Pingzhao Zhang
human	IHC	Am J Pathol; A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression.; Authors - Agell Laia L
rat	WB	Evid Based Complement Alternat Med; Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.; Authors - Chong-Chong Guo
human	WB	bioRxiv; The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation; Authors - Magda Wąchalska
human	WB	bioRxiv; Allosteric degraders induce CRL5 ASB8 mediated degradation of XPO1; Authors - Casey Elizabeth Hudman-Wing

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