Anticorps Monoclonal anti-TCF12

TCF12 Monoclonal Antibody for Indirect ELISA, WB

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

Indirect ELISA, WB

Conjugaison

Non conjugué

CloneNo.

1B8D4

N° de cat : 67523-1-PBS

Synonymes

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

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Galerie de données de validation

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  WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67523-1-Ig (same clone as 67523-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67523-1-Ig (same clone as 67523-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67523-1-Ig (same clone as 67523-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67523-1-PBS cible TCF12 dans les applications de Indirect ELISA, WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TCF12 Protéine recombinante Ag6287
• Nom complet: transcription factor 12
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC050556
• Symbole du gène: TCF12
• Identification du gène (NCBI): 6938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

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